Related Experiment Video

Updated: Feb 8, 2026

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice
08:50

Duodenal-Jejunal Bypass Surgery in Diet-Induced Obese Diabetic Mice

Published on: October 18, 2024

1.1K

Loss of Tbk1 kinase activity protects mice from diet-induced metabolic dysfunction

Victoria H Cruz1, Emily N Arner1, Katherine W Wynne1

  • 1Division of Surgical Oncology, Department of Surgery and the Hamon Center for Therapeutic Oncology Research, USA.

Molecular Metabolism
|June 25, 2018
PubMed
Abstract

Insights

Loss of TANK Binding Kinase 1 (TBK1) protects against metabolic dysfunction in mice fed an obesogenic diet. This protective effect is due to TBK1 no longer inhibiting the insulin receptor.

Area of Science:

  • Biochemistry
  • Metabolic disease research
  • Molecular biology

Background:

  • TANK Binding Kinase 1 (TBK1) is implicated in metabolic regulation.
  • Amlexanox, a dual IKKε/TBK1 inhibitor, shows promise for obesity and type 2 diabetes treatment.
  • The specific metabolic role of TBK1 remains unclear due to amlexanox's dual action.

Purpose of the Study:

  • To elucidate the unique metabolic functions of TBK1.
  • To investigate the metabolic profile of mice lacking TBK1 kinase function.
  • To identify mechanisms underlying TBK1's metabolic influence.

Main Methods:

  • Examined the metabolic profile of global Tbk1 mutant mice.
  • Challenged mice with an obesogenic diet.
  • Investigated the interaction between TBK1 and the insulin receptor.

Main Results:

  • Systemic loss of TBK1 kinase function conferred a protective metabolic phenotype in mice on an obesogenic diet.
  • This protection was mediated by the absence of an inhibitory interaction between TBK1 and the insulin receptor.
  • TBK1 kinase activity negatively impacts metabolic health.

Conclusions:

  • TBK1 plays a critical role in metabolic regulation.
  • Disrupting the TBK1-insulin receptor interaction may offer therapeutic benefits for metabolic diseases.
  • Targeting TBK1 could be a strategy for treating obesity and type 2 diabetes.
Keywords:
IKKεInsulinInsulin resistanceMetabolismObesityTBK1

More Related Videos

Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease with Fibrosis06:26

Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease with Fibrosis

Published on: July 18, 2025

888
An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment10:31

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment

Published on: April 20, 2018

11.2K

Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.2K
Line Loss01:10

Line Loss

The different configurations of source-load connections include wye (star) and delta connections. The relationship between line and phase voltages and currents varies depending on the configuration. When the source is supplying power, it is transmitted through the wires to the load, and during this transmission, some power is absorbed by the wires, leading to line loss.
Line loss impacts power delivery efficiency in a balanced three-phase circuit. The symmetry in such a circuit simplifies the...
545
What is Metabolism?00:52

What is Metabolism?

Overview
132.0K
Reducing Line Loss01:18

Reducing Line Loss

In a three-phase circuit, line loss is an indicator of energy dissipated as heat due to the resistance of transmission lines. To address this, incorporating transformers into the system—a step-up transformer at the source and a step-down transformer at the load—is a strategic solution. Two three-phase transformers are introduced to improve this.
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss in...
390
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
18.9K
Protection of Alcohols02:31

Protection of Alcohols

This lesson delves into the concept of protection and deprotection of a functional group fundamental to synthetic organic chemistry. These phenomena are explained in the context of aliphatic and aromatic alcohols.
Protection
It defines a protecting group as the masking agent to make the more reactive species inert to a given set of conditions. This concept is depicted via the illustration of liquid flow through different outlets in an assembly of pipes. The analogy helps to understand the role...
8.1K