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Updated: Feb 8, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
The influence of phosphorus on the autotrophic and mixotrophic denitrification
Chunzhen Fan1, Peiqi Wang2, Weili Zhou3
1School of Environmental Science and Engineering, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China; School of Agriculture and Biology, Shanghai Jiaotong University, No. 800, Dongchuan Road, Minhang District, Shanghai 200240, China.
Abstract:
Autotrophic and mixotrophic denitrification, two approaches of biological denitrification, have drawn more and more attention among the techniques to remove nitrogen from the aquatic environment. This study investigated the influence of phosphorus on the denitrification performance and bacterial community structure in the autotrophic and mixotrophic denitrification reactors. The activity test was applied to evaluate the variation of denitrification activity of autotrophic and mixotrophic sludge before and after phosphorus addition. High-throughput sequencing was used to analyze the change of bacterial community structure. The results showed that NO3--N removal efficiency of autotrophic and mixotrophic denitrification process increased by 40 and 35%, respectively, after phosphorus addition. The sludge denitrification activity of autotrophic and mixotrophic sludge was enhanced significantly. And phosphorus addition could greatly improve the proportion of denitrifying bacteria in both autotrophic (from 11.83 to 64.31%) and mixotrophic denitrifying sludge (from 13.59 to 45.12%). Overall, phosphorus addition could greatly improve the autotrophic and mixotrophic denitrification ability in the phosphorus deficient surface water.
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