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Chromosomal assignment of two endogenous ecotropic murine leukemia virus proviruses of the AKR/J mouse strain
Abstract:
The AKR/J mouse strain is genetically fixed for three different ecotropic murine leukemia virus genomes, designated Akv-1, Akv-3, and Akv-4 (Emv-11, Emv-13, and Emv-14). With recombinant inbred strains and crosses with linkage-testing stocks, Akv-3 and Akv-4 were placed on the mouse chromosome map. Akv-3, which encodes a replication-defective provirus, maps near the agouti coat color locus, a, on chromosome 2. Akv-4, which is replication competent, maps near the neurological mutant gene locus trembler, Tr, on chromosome 11. Akv-1 and Akv-2 (Emv-12), an ecotropic provirus carried by AKR/N but not AKR/J, have previously been mapped to chromosome 7 and 16, respectively. Thus, the four Akv proviruses mapped to date are on four different chromosomes. Akv-3 is the second ecotropic murine leukemia virus provirus to be mapped near the agouti locus. The results are discussed in relation to possible nonrandomness of viral integration.
Insights
Researchers mapped two murine leukemia virus (MLV) proviruses, Akv-3 and Akv-4, to specific mouse chromosomes. Akv-3 is near the agouti locus on chromosome 2, and Akv-4 is near the trembler locus on chromosome 11.
Area of Science:
- Genetics
- Virology
- Mouse models
Background:
- The AKR/J mouse strain harbors three ecotropic murine leukemia virus (MLV) genomes: Akv-1, Akv-3, and Akv-4.
- Previous research had mapped Akv-1 and Akv-2 (Emv-12) to chromosomes 7 and 16, respectively.
Purpose of the Study:
- To map the Akv-3 and Akv-4 MLV proviruses within the mouse genome.
- To investigate the chromosomal locations of these endogenous viral elements.
Main Methods:
- Utilized recombinant inbred strains and linkage-testing crosses in mice.
- Genetic mapping techniques were employed to determine the chromosomal positions of Akv-3 and Akv-4.
Main Results:
- Akv-3, a replication-defective provirus, was mapped to mouse chromosome 2, near the agouti (a) coat color locus.
- Akv-4, a replication-competent provirus, was mapped to mouse chromosome 11, near the trembler (Tr) neurological mutant gene locus.
- All four mapped Akv proviruses (Akv-1, Akv-2, Akv-3, Akv-4) are located on distinct chromosomes.
Conclusions:
- The Akv-3 provirus is the second ecotropic MLV provirus identified near the agouti locus.
- The distinct chromosomal locations of these MLV proviruses suggest potential nonrandomness in viral integration events.
- Findings contribute to understanding the genetic basis and integration patterns of endogenous retroviruses in mice.