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Updated: Feb 8, 2026
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Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
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Changes in erythrocyte ATPase activity under different pathological conditions.
Ali A Kherd1, Nawal Helmi1,2, Khadijah Saeed Balamash1
1Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
African Health Sciences
|June 26, 2018
Summary
Na+-K+ ATPase activity in red blood cells can serve as a diagnostic biomarker for various physiological conditions. This enzyme
Area of Science:
- Biochemistry
- Physiology
- Biomarker Discovery
Background:
- Na+-K+ ATPase is crucial for active transport of ions in red blood cells.
- Its activity is altered in disrupted cell membranes.
- The enzyme functions as a Na+-K+ pump, utilizing ATP hydrolysis.
Purpose of the Study:
- To investigate Na+-K+ ATPase activity as a potential biomarker.
- To assess its diagnostic utility in various physiological and pathological states.
Main Methods:
- Determined Na+-K+ ATPase activity in blood samples.
- Compared activity across conditions: pregnancy, smoking, diabetes, renal dysfunction, and healthy controls.
- Matched subjects for age and sex.
Main Results:
- Elevated Na+-K+ ATPase activity observed in pregnancy, smoking, diabetes, and kidney disease.
- Significantly higher activity compared to healthy individuals.
- Specific activity values provided for each condition.
Conclusions:
- Na+-K+ ATPase specific activity is a viable biomarker for diagnosing physiological diseases.
- Demonstrates potential for clinical diagnostic applications.
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