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Related Experiment Videos

ISL1: a new transposable element in Lactobacillus casei.

M Shimizu-Kadota, M Kiwaki, H Hirokawa

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary
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    Researchers identified a transposable element, ISL1, in Lactobacillus phage genomes. This 1.3 kbp DNA insertion was found in virulent mutants and exhibits characteristics of mobile genetic elements.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genomics

    Background:

    • Temperate Lactobacillus phages are crucial for understanding bacterial genetics and phage-bacterium interactions.
    • Virulent mutants of phages can provide insights into genetic changes driving host range and pathogenicity.

    Purpose of the Study:

    • To elucidate the genome structures of the temperate Lactobacillus phage phi FSW and its virulent mutants, phi FSVs.
    • To identify and characterize genetic elements responsible for the differences between the temperate phage and its virulent mutants.

    Main Methods:

    • Restriction enzyme analysis of phage DNA.
    • Heteroduplex analysis to compare DNA structures.
    • Nucleotide sequence analysis to determine precise genetic makeup.

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    Main Results:

    • Two of three virulent phi FSV mutants contained a 1.3 kbp insertion, designated ISL1, at varying locations within the phi FSW genome.
    • ISL1 is 1,256 bp long, featuring two significant open reading frames and terminal inverted repeats.
    • ISL1 was flanked by 3 bp direct repeats of the host phi FSW sequence, indicating its insertion mechanism.

    Conclusions:

    • The ISL1 element exhibits key characteristics of a transposable element.
    • ISL1 is identified as a mobile genetic element within the Lactobacillus casei phage system.
    • The insertion of ISL1 likely contributes to the genetic variation observed in the virulent phage mutants.