Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.4K
Binary Fission01:26

Binary Fission

3.2K
Binary fission is the primary mode of asexual reproduction in prokaryotes, such as bacteria. It results in the production of two genetically identical daughter cells. This highly efficient process ensures the rapid propagation of bacterial populations under favorable conditions and involves coordinated cellular and molecular events.DNA Replication and SeparationThe process begins with the replication of the bacterial chromosome. The circular DNA molecule unwinds at a specific origin of...
3.2K
Binary Fission01:20

Binary Fission

64.0K
Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
64.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
From DNA to Protein03:06

From DNA to Protein

22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selenium-GPX4 axis orchestrates intestinal arachidonic acid metabolic reprogramming to mitigate inflammation.

Free radical biology & medicine·2026
Same author

Quantitative analysis of colorimetric hydrogel/paper mini-disk arrays using a handheld Wi-Fi scanner.

The Analyst·2026
Same author

Elucidating key lipids driving aroma differences and potential formation pathways in marbled beef with interindividual lipid variations.

Food chemistry·2026
Same author

Genotype-phenotype correlations of fasting C-peptide and lipids in HNF1A-MODY: a single-center series and multi-center cross-sectional analysis in Chinese population.

Frontiers in endocrinology·2026
Same author

Combining the Pitcher and Lotus Plant: Supericephobic and Superhydrophobic Silicone Films.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Multi-omics analysis of lipids and aroma compounds in beef under grain-fed and grass-fed production methods.

Current research in food science·2025

Related Experiment Video

Updated: Feb 8, 2026

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.9K

Binary Thiolate DNA/Ferrocenyl Self-Assembled Monolayers on Gold: A Versatile Platform for Probing Biosensing

Lin Qi1, Huihui Tian1,2, Hua-Zhong Yu1

  • 1Department of Chemistry , Simon Fraser University , Burnaby , British Columbia V5A 1S6 , Canada.

Analytical Chemistry
|June 26, 2018
PubMed
Summary

This study developed DNA/ferrocene biosensing interfaces for electrochemical characterization. These DNA self-assembled monolayers provide quantitative insights into DNA film properties for improved biosensor performance.

More Related Videos

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
10:27

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Published on: August 25, 2009

11.9K
Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
09:17

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

9.2K

Related Experiment Videos

Last Updated: Feb 8, 2026

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
14:18

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Published on: October 4, 2011

14.9K
Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
10:27

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Published on: August 25, 2009

11.9K
Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
09:17

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

9.2K

Area of Science:

  • Electrochemistry
  • Biosensor Technology
  • Surface Science

Background:

  • DNA self-assembled monolayers (SAMs) are crucial for biosensor performance.
  • Understanding DNA film properties is key to optimizing DNA-analyte binding.
  • Current methods for characterizing DNA SAMs can be limited.

Purpose of the Study:

  • To prepare and electrochemically characterize binary thiolate DNA/6-ferrocenyl-1-hexanethiol (FcC6SH) SAMs on gold (DNA/FcC6S-Au).
  • To demonstrate the use of redox-active thiols for quantitative analysis of DNA film properties.
  • To establish a versatile platform for DNA-based biosensing interfaces.

Main Methods:

  • Fabrication of binary DNA/FcC6SH SAMs on gold substrates.
  • Electrochemical characterization using cyclic voltammetry (CV).
  • Analysis of redox responses of surface-tethered ferrocene (Fc) and [Ru(NH3)6]3+.

Main Results:

  • CV studies confirmed Fc redox responses provide quantitative DNA film data.
  • Redox probes revealed surface density, structural heterogeneity, and molecular orientation.
  • The DNA/FcC6S-Au SAM system allowed for detailed property analysis under varied conditions.

Conclusions:

  • Redox-active thiols are effective for passivating and labeling DNA biosensing interfaces.
  • This approach offers a convenient method for electrochemical characterization of DNA SAMs.
  • The developed system enhances the performance and data analysis of DNA-based biosensors.