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MicroRNA-383 acts as a tumor suppressor in colorectal cancer by modulating CREPT/RPRD1B expression

Jian Li1, Amber R Smith2, Rebecca T Marquez2

  • 1School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei Province, China.

Molecular Carcinogenesis
|June 26, 2018
PubMed

Insights

MicroRNA-383 (miR-383) suppresses colorectal cancer growth by inhibiting Cell-cycle-related and expression-elevated protein in tumor (CREPT) expression. Decreased miR-383 levels contribute to high CREPT expression in tumors, suggesting miRNA-based therapies.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Cell-cycle-related and expression-elevated protein in tumor (CREPT) promotes cell proliferation and is highly expressed in various tumors.
  • The regulatory mechanisms of CREPT in colorectal cancer (CRC) remain largely unknown.
  • MicroRNAs (miRNAs) are critical regulators of gene expression implicated in cancer development.

Purpose of the Study:

  • To investigate the role of miR-383 in regulating CREPT expression in colorectal cancer.
  • To elucidate the functional consequences of miR-383/CREPT interaction on CRC cell behavior and tumor growth.

Main Methods:

  • Quantitative real-time PCR and Western blotting to assess CREPT and miR-383 expression levels.
  • Luciferase reporter assays to confirm direct targeting of CREPT by miR-383.
  • Cell proliferation, colony formation, and in vivo tumor growth assays in response to miR-383 modulation.

Main Results:

  • CREPT was upregulated, while miR-383 was downregulated in CRC cell lines and tissues.
  • miR-383 directly targeted the 3'-UTR of CREPT mRNA, inhibiting its expression at both mRNA and protein levels.
  • Overexpression of miR-383 suppressed CRC cell proliferation, colony formation, and tumor growth, accompanied by decreased CREPT levels.

Conclusions:

  • The study identifies miR-383 as a negative regulator of CREPT in colorectal cancer.
  • Decreased miR-383 levels contribute to the aberrant overexpression of CREPT in CRC.
  • miR-383 represents a potential therapeutic target for colorectal cancer treatment.
Keywords:
CREPTcolony formationcolorectal cancermiR-383tumor growth

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