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Related Experiment Video

Updated: Feb 8, 2026

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
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Expansion microscopy: enabling single cell analysis in intact biological systems.

Shahar Alon1,2, Grace H Huynh1,2,3, Edward S Boyden1,2,4,5,6

  • 1Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.

The FEBS Journal
|June 26, 2018
PubMed
Summary

Expansion microscopy (ExM) enables detailed single-cell analysis by magnifying tissues for super-resolution imaging. This powerful technique allows researchers to identify biomolecules and understand cell relationships within intact biological systems.

Keywords:
FISHexpansion microscopygenomicsmorphologymultiplexingsingle cell analysissuper-resolution microscopy

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biochemistry

Background:

  • Current single-cell analysis methods lack the resolution and scale to study biomolecules within intact tissues.
  • Identifying and localizing biomolecules is crucial for classifying cells and understanding their interactions in complex biological systems.

Purpose of the Study:

  • To highlight the potential of Expansion Microscopy (ExM) for advanced single-cell analysis.
  • To demonstrate ExM's capability in integrating molecular information with cellular morphology at high spatial resolution.

Main Methods:

  • Expansion Microscopy (ExM) physically magnifies biological samples isotropically.
  • Utilizes diffraction-limited microscopes to achieve super-resolution imaging.
  • Enables rapid image acquisition over a large field of view.

Main Results:

  • ExM provides the necessary spatial precision to resolve individual biological components.
  • The technology offers the scale and accessibility required for analyzing large 3D biological structures like tissues and organs.
  • ExM integrates molecular content and cellular morphology effectively.

Conclusions:

  • Expansion Microscopy is a powerful tool for high-resolution, large-scale imaging of cells and tissues.
  • ExM facilitates the characterization and classification of individual cells and their spatial relationships within intact systems.
  • This technology addresses the need for advanced single-cell analysis methods in biological research.