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Updated: Feb 8, 2026

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform
Published on: August 15, 2017
A simple microfluidic device for live cell imaging of Arabidopsis cotyledons, leaves, and seedlings
Shia Vang1, Kati Seitz2, Patrick J Krysan1,3
1Horticulture Department, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA.
Abstract:
One of the challenges of performing live-cell imaging in plants is establishing a system for securing the sample during imaging that allows for the rapid addition of treatments. Here we report how a commercially available device called a HybriWell™ can be repurposed to create an imaging chamber suitable for Arabidopsis seedlings, cotyledons and leaves. Liquid in the imaging chamber can be rapidly exchanged to introduce chemical treatments via microfluidic passive pumping. When used in conjunction with fluorescent biosensors, this system can facilitate live-cell imaging studies of signal transduction pathways triggered by different treatments. As a demonstration, we show how the HybriWell can be used to monitor flg22-induced calcium transients using the R-GECO1 calcium indicator in detached Arabidopsis leaves.
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