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Updated: Feb 8, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
Impact of the Keto-Enol Tautomeric Equilibrium on the BODIPY Chromophore
Volker Leen1, Marina Laine2, Joseph Molisho Ngongo3
1Department of Chemistry , KU Leuven , Celestijnenlaan 200f-bus 02404 , 3001 Leuven , Belgium.
Abstract:
An intramolecular tautomeric fluorescent BODIPY sensor has been designed and synthesized. The obtained BODIPY dye is a combination of the 4-bora- 3a, 4a-diaza- s-indacene core and a diketone fragment. The study of conformational equilibria in the ground and excited states has been completed for a broad range of solvent polarity by steady state and NMR methods as well as by DFT and TD-DFT calculations. The interpretation of the unique emission observed in hydrogen bond accepting solvents upon the excitation of the fluorescent dye in the S0-S2 transition has been accomplished. The Jablonski diagram has been analyzed for the observed processes in the BODIPY dye studied on the basis of DFT and TD-DFT calculations.
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