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Related Experiment Video

Updated: Feb 8, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

Inositol polyphosphates regulate and predict yeast pseudohyphal growth phenotypes.

Kaitlyn L Norman¹, Christian A Shively², Amberlene J De La Rocha¹

¹Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America.

|June 26, 2018

Summary

This summary is machine-generated.

Inositol pyrophosphates (InsPs) regulate yeast pseudohyphal growth. Elevated 5PP-InsP5 levels serve as a marker for this filamentous growth, influenced by key kinases.

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Area of Science:

Cellular Biology
Biochemistry
Microbiology

Background:

Pseudohyphal growth in budding yeast forms multicellular filaments, crucial for fungal virulence and stress response.
While genetics of pseudohyphal growth are known, metabolite changes driving filamentation remain unclear.
Inositol polyphosphates (InsPs) are key signaling metabolites involved in cellular processes.

Purpose of the Study:

Investigate the role of inositol pyrophosphate (InsP) biosynthesis in yeast pseudohyphal growth.
Identify specific InsP isoforms and their correlation with filamentation.
Determine the relationship between pseudohyphal growth kinases and InsP signaling.

Main Methods:

Utilized deletion and overexpression analyses of InsP kinases in yeast.
Analyzed inositol pyrophosphate profiles under nitrogen-limiting and normal conditions.
Assessed pseudohyphal growth phenotypes in kinase deletion mutants.

Main Results:

The InsP biosynthesis pathway is essential for wild-type pseudohyphal growth.
Elevated 5-pyrophosphorylated inositol pentakisphosphate (5PP-InsP5) relative to 1-pyrophosphorylated inositol pentakisphosphate (1PP-InsP5) correlates with hyper-filamentous growth.
Overexpression of KCS1 induced pseudohyphal growth, and key kinases (Snf1p, Kss1p, Fus3p) are required for both wild-type InsP levels and pseudohyphal growth.

Conclusions:

Elevated 5PP-InsP5 levels serve as an in vivo marker for hyper-filamentous growth in yeast.
Pseudohyphal growth kinases regulate inositol pyrophosphate signaling.
This study provides novel insights into the metabolic regulation of filamentous growth.