Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Type II restriction endonucleases cleave single-stranded DNAs in general.

K Nishigaki, Y Kaneko, H Wakuda

Nucleic Acids Research

|August 26, 1985

Summary

Restriction enzymes can cleave single-stranded DNA (ssDNA) by targeting specific secondary structures. The cleavage rate depends on the stability of these transiently formed canonical structures within the ssDNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular survey of Bartonella henselae and Bartonella clarridgeiae in pet cats across Japan by species-specific nested-PCR.

Epidemiology and infection·2017

Same author

Change in the Contents of Arginine, Ornithine, and Urea in the Muscle of Marine Invertebrates Stored in Ice.

Bioscience, biotechnology, and biochemistry·2016

Same author

Visualization of internal carotid artery atherosclerotic plaques in symptomatic and asymptomatic patients: a comparison of optical coherence tomography and intravascular ultrasound.

AJNR. American journal of neuroradiology·2011

Same author

In vitro selective suppression of feline myeloid colony formation is attributable to molecularly cloned strain of feline leukemia virus with unique long terminal repeat.

Research in veterinary science·2004

Same author

[Is minimal skin incision and partial sternotomy approach for congenital heart defects less invasive?; evaluation of SIRS on ventricular septal defect].

Kyobu geka. The Japanese journal of thoracic surgery·2002

Same author

Noninvasive quantitative tissue characterization and two-dimensional color-coded map of human atherosclerotic lesions using ultrasound integrated backscatter: comparison between histology and integrated backscatter images.

Journal of the American College of Cardiology·2001

Area of Science:

Molecular Biology
Enzymology

Background:

Restriction endonucleases are enzymes traditionally known for cleaving double-stranded DNA (dsDNA) at specific recognition sites.
The ability of these enzymes to interact with and cleave single-stranded DNA (ssDNA) has been less explored.

Purpose of the Study:

To investigate the capability of various restriction endonucleases to cleave ssDNA.
To propose and support a model explaining the mechanism of ssDNA cleavage by restriction enzymes.

Main Methods:

Testing a panel of 18 restriction endonuclease species for their ability to cleave ssDNA.
Developing a theoretical model based on enzyme recognition sequences and DNA secondary structures.

Main Results:

13 out of 18 tested restriction enzymes demonstrated the ability to cleave ssDNA.

Related Experiment Videos

New evidence reported for AvaII, HaeII, DdeI, AluI, Sau3AI, AccII, TthHB8I, and HapII cleaving ssDNA.

A model was proposed where enzymes cleave transient secondary structures (canonical structures) in ssDNA, dependent on recognition sequence symmetry and structure stability.

Conclusions:

Restriction enzymes possess a broader substrate range than previously thought, including ssDNA.
The cleavage of ssDNA by restriction enzymes is facilitated by transient secondary structures, offering insights into enzyme-substrate interactions and potential applications.