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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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MicroRNA-126 attenuates cell apoptosis by targeting TRAF7 in acute myeloid leukemia cells

Qian Ding1,1, Qing Wang1,1, Yi Ren1,1

  • 1Department of Hematology, Gui Zhou Provincial People's Hospital, Guiyang 550002, P.R. China.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|June 26, 2018
PubMed

Insights

MicroRNA-126 (miRNA-126) promotes acute myeloid leukemia (AML) survival by down-regulating TRAF7, inhibiting apoptosis. Inhibiting miRNA-126 offers a potential therapeutic strategy for AML patients.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Acute myeloid leukemia (AML) exhibits poor prognosis, largely due to leukemia stem-like cells (LSCs) with self-renewal and differentiation capabilities.
  • Identifying novel therapeutic targets within LSCs is crucial for improving AML patient outcomes.

Purpose of the Study:

  • To investigate the roles of microRNA-126 (miRNA-126) and tumor necrosis factor receptor-associated factor 7 (TRAF7) in AML.
  • To elucidate the mechanism by which miRNA-126 influences AML cell proliferation and apoptosis.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot to assess miRNA-126 and TRAF7 expression.
  • MTT assay for cell proliferation and flow cytometry for apoptosis analysis.
  • Dual-luciferase assay to identify miRNA-126 targets and confirm the TRAF7-mediated apoptosis suppression pathway.

Main Results:

  • miRNA-126 was highly expressed across all investigated AML cell lines.
  • Inhibition of miRNA-126 significantly induced apoptosis and cell death in AML cells.
  • High miRNA-126 expression suppresses apoptosis by down-regulating TRAF7, which subsequently blocks the c-FLIP pathway.

Conclusions:

  • miRNA-126 plays a significant role in AML pathogenesis by promoting cell survival.
  • Targeting miRNA-126 presents a promising therapeutic strategy to enhance apoptosis and improve clinical outcomes in AML.
Keywords:
TRAF7acute myeloid leukemia cellsapoptoseapoptosiscellules de leucémie myéloïde aigüemiARN-126miRNA-126

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