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Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
Published on: October 10, 2025
Expression and significance of Ki-67 in lung cancer
Roxana Folescu1, Codrina Mihaela Levai, Mirela Loredana Grigoraş
1Discipline of Anatomy and Embryology, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania; mirela.grigoras@yahoo.com; Department of Pediatrics, "Victor Babes" University of Medicine and Pharmacy, Timisoara, Romania; sarghirescu@yahoo.com.
Abstract:
Ki-67 parameter is a proliferation marker in malignant tumors. The increased proliferation activity and the decreased prognosis in lung cancer determined us to investigate different parameters connected to the tumor's aggression, such as cellularity, Ki-67 positivity rate, and proliferating cell nuclear antigen (PCNA). We evaluated the proliferative activity in 62 primary lung tumors by determining the cell's percentage of Ki-67 and immunoreactive PCNA (using MIB-1 and PCNA monoclonal antibodies), classifying Ki-67 and PCNA immunoreactivity into three score groups. The results obtained emphasized a linkage between Ki-67 score with the histological tumor subtype, tumor cellularity and degree of differentiation and with other proliferation immunohistochemistry (IHC) markers, such as p53 cellular tumor antigen. The tumor's cellularity, the Ki-67 positivity rate and PCNA, together with the clinical stage and the histological differentiation bring extra pieces of useful information in order to anticipate the evolution and the prognosis of lung cancer.
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