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Updated: Feb 8, 2026

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
Published on: April 4, 2018
The study of bacteria in biopsies from Slovak colorectal adenoma and carcinoma patients
L Wachsmannova1, J Majek2, V Zajac1
1Department of Genetics, Cancer Research Institute BMC, Slovak Academy of Sciences, Bratislava, Slovakia
Abstract:
The development of colorectal cancer is affected by many factors, especially the intestinal microbiota. However, precise knowledge of bacterial communities associated with the mucosa in various parts of the colon is limited. Herein, we applied the gentamicin protection assay and detected the presence of intracellular bacteria in colorectal biopsies from Slovak patients with colorectal adenoma and carcinoma, and we compared this with healthy controls. The ENTEROtest 24 and MALDI-TOF mass spectrometry identified the cultivated bacteria and results revealed the presence of intracellularly localized Escherichia coli, Proteus mirabilis and Proteus vulgaris in patients with colorectal adenomas and carcinomas. In addition to these species, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus were identified in colorectal biopsies, but these were extracellular. The marked increase in relative abundance of intracellular E. coli in patients with colorectal adenomas and carcinomas was statistically significant compared to controls, and our preliminary data supports E. coli's role as a pro-oncogenic pathogen.
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