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Updated: Jul 31, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
[Potential activity of the external pathway of NADH oxidation in mitochondria]
Abstract:
High rate of exogenic NADH oxidation (up to 200 mg-at. oxygen for 1 min per 1 mg of protein and higher) along the rothenone, antimycin-nonsensitive pathway is observed under interaction of mitoplasts with the external membrane and cytochrome c. In the medium with low ionic strength the interaction of external and internal membranes is not a sufficient condition for activating the external pathway of the NADH oxidation: the presence of exogenic cytochrome c is also necessary. With saturated cytochrome c concentrations the addition of outer membranes leads to further stimulation of the NADH oxidation. In the medium with high ionic strength external membranes stimulate oxidation of NADH when exogenic cytochrome c is absent; the subsequent addition of cytochrome c stimulates the NADH oxidation in this medium to a greater extent than in the medium with the low ionic strength. Under the nonlimited interaction of external and internal membranes and cytochrome c the potential activity of the outer pathway of NADH oxidation in the liver mytoplasts of hybernating gophers is lower than in the liver mytoplasts of rats.
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