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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Published on: December 27, 2016

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Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of

Apichakan Sampannang1, Supatcharee Arun1, Jaturon Burawat1

  • 1Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

International Journal of Reproductive Biomedicine
|June 27, 2018
PubMed
Abstract

Insights

Diabetes mellitus in males causes testicular damage and infertility. This study shows that streptozotocin-induced diabetes in mice leads to abnormal sperm function and altered protein expression, clarifying potential infertility mechanisms.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Toxicology

Background:

  • The streptozotocin (STZ)-induced diabetic model is crucial for studying diabetes mellitus (DM) effects on male reproductive health.
  • Understanding the precise mechanisms of DM-induced male sub/infertility remains a critical research gap.

Purpose of the Study:

  • To investigate testicular histopathology, sperm acrosome reaction (AR) status, and tyrosine-phosphorylated protein expression in STZ-induced diabetic mice.
  • To elucidate the cellular and molecular changes contributing to male infertility in a diabetic model.

Main Methods:

  • Male ICR mice were divided into control and multiple low-dose streptozotocin (MLD-STZ) induced diabetes groups.
  • Evaluated reproductive parameters, sperm acrosome reaction (AR), testicular/epididymal histopathology, and testicular tyrosine-phosphorylated protein expression.
  • Diabetes was induced via daily STZ injections for five consecutive days.

Main Results:

  • STZ-induced diabetes significantly increased blood glucose, sperm abnormality, and AR percentages, while decreasing sperm concentration.
  • Testicular histopathology revealed significant alterations in seminiferous tubules and the presence of round cells in the epididymis.
  • Markedly increased expression of specific testicular phosphorylated proteins (170, 70, 36, 30, 25 kDa) and decreased expression of a 120 kDa protein were observed.

Conclusions:

  • MLD-STZ-induced diabetes leads to diverse testicular histopathologies and precocious sperm AR in male mice.
  • Alterations in testicular protein phosphorylation patterns are associated with STZ-induced diabetes.
  • These findings provide insights into the mechanisms underlying male sub/infertility in diabetes mellitus.
Keywords:
Diabetes mellitusMicePhosphorylated proteinStreptozotocin

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