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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

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Dendritic cell trafficking in tumor-bearing mice

Sarah C Krzastek1, Ekaterine Goliadze1, Shaoqing Zhou1

  • 1Division of Urology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Cancer Immunology, Immunotherapy : CII
|June 27, 2018
PubMed

Insights

Optimizing dendritic cell (DC) therapy for prostate cancer requires understanding DC trafficking. Subcutaneous delivery of antigen-loaded DCs shows the most effective immune cell migration to lymph nodes and tumors.

Area of Science:

  • Immunology
  • Oncology
  • Biotechnology

Background:

  • Prostate cancer is a leading cause of cancer mortality with limited treatment options for metastatic disease.
  • Current immunotherapies show restricted efficacy.
  • Dendritic cells (DCs) are crucial for initiating anti-tumor immune responses by priming T cells in lymphoid organs.

Purpose of the Study:

  • To investigate in vivo dendritic cell (DC) trafficking following different administration routes.
  • To optimize DC-based therapy for prostate cancer by determining the most effective delivery method.
  • To evaluate the impact of antigen loading on DC migration patterns.

Main Methods:

  • Developed a novel in vivo fluorescent imaging system using 1,1'-dioctadecyltetramethyl indotricarbocyanine Iodine to track labeled DCs.
  • Administered mature, labeled DCs intravenously, subcutaneously, or intratumorally into a murine prostate tumor model (RM-1 cells).
  • Compared DC trafficking and signal intensity in tumors and lymphoid organs with and without antigen loading (whole tumor lysate).

Main Results:

  • All administration routes demonstrated DC trafficking to the tumor site.
  • Subcutaneous injection showed preferential migration to lymph nodes and the tumor.
  • Intravenous injection resulted in DC accumulation in lungs, intestines, and spleen.
  • Subcutaneous delivery of antigen-pulsed DCs yielded the highest signal intensity in tumors and lymph nodes.

Conclusions:

  • Subcutaneous administration of antigen-loaded DCs enhances preferential trafficking to key immune organs.
  • This optimized delivery strategy holds promise for improving the efficacy of DC-based immunotherapy for prostate cancer.
  • Understanding DC migration patterns is critical for designing effective cancer treatments.
Keywords:
CITIM 2017Cell traffickingDendritic cellsFluorescent imagingNear-infrared dyeProstate cancer

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