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[Effects of recombinant human interferon β1a on the chondrogenic differentiation of human bone marrow mesenchymal

Qiaoqiao Li1, Lijun Zhang2, Zhili Huang2

  • 1School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, Guangdong, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 27, 2018
PubMed

Recombinant human interferon beta (rhIFN-β) is a glycoprotein produced by genetically engineered cells and has anti-virus, anti-tumor and immunoregulation functions. Although studies have shown that other subtypes of IFN such as IFN-γ affects cell proliferation and differentiation to some extent, the effect of rhIFN-β on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) is less known. In this study we studied the effect of rhIFN-β on the chondrogenic differentiation of hMSCs by inducing hMSCs into cartilage pellet via adding IFN-β1a into regular TGF-β3 chondrogenic differentiation medium. We collected the induced pellets and then detected GAG content, assessed pellets size, observed agreecan using alcian blue staining, and analyzed the expression of Sox and CollangenⅡusing real-time PCR and Western blotting. Addition of 100 ng/mL IFN-β1a to regular TGF-β3 chondrogenic differentiation medium could improve the concentration of GAG, increase the size of pellets, promote the formation of aggrecan and up-regulate the expression of CollangenII and Sox9. IFN-β1a combined with TGF-β3 could promote chondrogenic differentiation of hMSCs.

Keywords:
TGF-β3chondrogenic differentiationhMSCsrecombinant human interferon beta 1a

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