Long noncoding RNA LINC00858 promotes osteosarcoma through regulating miR-139-CDK14 axis

Area of Science:

• Molecular Biology
• Oncology
• Genetics

Background:

• Long noncoding RNAs (lncRNAs) are implicated in human cancer development.
• The specific role of lncRNAs in osteosarcoma oncogenesis remains unclear.

Purpose of the Study:

• To investigate the function and mechanism of long intergenic non-protein coding RNA 00858 (LINC00858) in human osteosarcoma tumorigenesis.

Main Methods:

• Quantitative real-time PCR to assess LINC00858 expression in osteosarcoma tissues and cell lines.
• In vitro and in vivo experiments to evaluate the effects of LINC00858 silencing on osteosarcoma.
• Luciferase reporter assays and RNA immunoprecipitation (RIP) to confirm the interaction between LINC00858 and miR-139.
• Western blotting to validate CDK14 as a target of miR-139.
• Rescue experiments to elucidate the LINC00858/miR-139/CDK14 pathway's role.

Main Results:

• LINC00858 expression was significantly upregulated in osteosarcoma tissues and cell lines.
• LINC00858 silencing inhibited osteosarcoma cell proliferation and invasion in vitro and tumor growth in vivo.
• LINC00858 directly sponges miR-139, and CDK14 is a target of miR-139.
• The LINC00858/miR-139/CDK14 axis was confirmed to influence osteosarcoma cell phenotypes.

Conclusions:

• The LINC00858/miR-139/CDK14 axis promotes osteosarcoma tumorigenesis.
• LINC00858 represents a potential therapeutic target for osteosarcoma.