Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxidation Numbers03:14

Oxidation Numbers

42.9K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.9K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Pyruvate Oxidation01:15

Pyruvate Oxidation

169.1K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
169.1K
Aging01:26

Aging

719
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
719
Oxidation-Reduction Reactions03:11

Oxidation-Reduction Reactions

75.8K
Oxidation–Reduction Reactions
75.8K
Feedback Loops01:01

Feedback Loops

64.5K
In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
64.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dentinal tubule penetration of a silicone-based endodontic sealer following N-acetyl cysteine intracanal medicament removal using ultrasonic agitation and laser activated irrigation - An <i>in vitro</i> study.

Journal of conservative dentistry and endodontics·2025
Same author

Association of New-Onset Atrial Fibrillation With All-Cause Mortality in COVID-19 Patients.

Cureus·2023
Same author

Adipocyte Na, K-ATPase Signaling Attenuates Experimental Uremic Cardiomyopathy.

Cellular and molecular biology (Noisy-le-Grand, France)·2023
Same author

Inhibition of Na/K-ATPase signaling Attenuates Steatohepatitis and Atherosclerosis in Mice Fed a Western Diet.

Cellular and molecular biology (Noisy-le-Grand, France)·2023
Same author

Retraction Note: The Na/K-ATPase oxidant amplification loop regulates aging.

Scientific reports·2022
Same author

Retraction Note: The Adipocyte Na/K-ATPase Oxidant Amplification Loop is the Central Regulator of Western Diet-Induced Obesity and Associated Comorbidities.

Scientific reports·2022

Related Experiment Video

Updated: Feb 8, 2026

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.9K

The Na/K-ATPase Oxidant Amplification Loop Regulates Aging.

Komal Sodhi1, Alexandra Nichols1, Amrita Mallick1

  • 1Departments of Medicine, Surgery, and Biomedical sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, USA.

Scientific Reports
|June 28, 2018
PubMed
Summary

The Na/K-ATPase oxidant amplification loop (NKAL) drives aging. Targeting NKAL with pNaKtide may offer a novel anti-aging strategy by reducing cellular damage and senescence.

More Related Videos

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K
A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

17.2K

Related Experiment Videos

Last Updated: Feb 8, 2026

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification
07:25

Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification

Published on: June 25, 2020

6.9K
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

1.3K
A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci
05:03

A Loop-mediated Isothermal Amplification LAMP Assay for Rapid Identification of Bemisia tabaci

Published on: October 29, 2018

17.2K

Area of Science:

  • Gerontology
  • Cellular Biology
  • Biochemistry

Background:

  • Aging is characterized by increasing oxidant injury.
  • The Na/K-ATPase oxidant amplification loop (NKAL) is a newly identified pathway potentially involved in aging.
  • Understanding NKAL's role is crucial for developing anti-aging interventions.

Purpose of the Study:

  • To investigate the involvement of NKAL in the aging process.
  • To evaluate the potential of antagonizing NKAL as an anti-aging strategy.

Main Methods:

  • Utilized aged C57Bl6 mice fed a western diet or treated with pNaKtide.
  • Exposed human dermal fibroblasts (HDFs) to various oxidant stressors in vitro.
  • Assessed senescence markers, cell injury, apoptosis, and NKAL activity.
  • Compared the effects of pNaKtide with antioxidants N-Acetyl Cysteine and Vitamin E.

Main Results:

  • A western diet accelerated aging phenotypes in mice, while pNaKtide attenuated these effects.
  • Oxidant stress in HDFs stimulated NKAL, increased senescence, injury, and apoptosis.
  • Ouabain augmented senescence, whereas pNaKtide treatment attenuated it.
  • pNaKtide provided superior protection against senescence compared to N-Acetyl Cysteine and Vitamin E, particularly by reducing nuclear oxidant stress.

Conclusions:

  • The Na/K-ATPase oxidant amplification loop (NKAL) plays a significant role in aging.
  • Targeting NKAL with agents like pNaKtide shows promise as an effective anti-aging intervention.
  • pNaKtide demonstrates specific efficacy in mitigating nuclear oxidant stress and cellular senescence.