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Time Series Analysis of the Microbiota of Children Suffering From Acute Infectious Diarrhea and Their Recovery After

Ener C Dinleyici1, Daniel Martínez-Martínez2, Ates Kara3

  • 1Department of Pediatrics, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey.

Frontiers in Microbiology
|June 28, 2018
PubMed

Insights

Probiotic treatment with Saccharomyces boulardii helped children recover from rotavirus diarrhea by restoring gut microbiota diversity. This study tracked microbiota changes over 30 days, revealing a shift from a diseased to a healthy state.

Area of Science:

  • Microbiology
  • Pediatrics
  • Gastroenterology

Background:

  • Acute infectious diarrhea is a major global health issue in children.
  • Gut microbiota plays a crucial role in diarrhea and recovery.
  • Understanding microbiota dynamics during recovery is clinically important.

Purpose of the Study:

  • To correlate gut microbiota dynamics with the recovery of children suffering from rotavirus-induced acute infectious diarrhea.
  • To evaluate the effect of Saccharomyces boulardii CNCM I-745 on microbiota recovery.
  • To analyze temporal stability and composition changes in the gut microbiota during and after diarrhea.

Main Methods:

  • Study included 10 children with rotavirus diarrhea and 6 healthy controls (3-4 years old).
  • Rotavirus-infected children received Saccharomyces boulardii CNCM I-745 for 5 days.
  • Fecal samples were collected at multiple time points (0, 3, 5, 10, 30 days) for 16S rRNA gene sequencing, diversity analysis, and dynamical analysis (Taylor's law).

Main Results:

  • All rotavirus-infected children ceased diarrhea by day 3 post-intervention.
  • Alpha diversity was low in the first 5 days, increasing by days 10 and 30.
  • Gut microbiota composition differed between infected and healthy children initially, but normalized by the end of the study. Gammaproteobacteria abundance decreased with recovery.

Conclusions:

  • Saccharomyces boulardii CNCM I-745 administration facilitated the transition of gut microbiota from a diseased to a healthy state in children with rotavirus diarrhea.
  • Time-series analysis of gut microbiota is crucial for understanding and characterizing dysbiosis related to diarrhea.
  • The study provides valuable clinical insights into microbiota recovery dynamics post-diarrhea.

Keywords:
acute infectious diarrheamicrobiotarotavirussystems biologytemporal analysis

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