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Updated: Feb 8, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
[Effect of overdose fluoride on the expression of TGF-β3 in rat incisor]
1Department of Stomatology, Shanghai No.8 People's Hospital. Shanghai 201500, China.
Purpose:
To observe the effect of overdose fluoride on the expression of TGF-β3 in rat incisor and to explore the possible mechanism of dental fluorosis. METHODS: Twenty Wistar rats were randomly divided into 2 groups. The animals were maintained in standard environmental conditions with free access to food and water (control group) or water added with 100mg/L F (experimental group). The rats were killed at the end of 8th week. The expression of TGF-β3 was detected by immunohistochemical staining. Statistical analysis was performed using SPSS12.0 software package.
Results:
The expression of TGF-β3 in ameloblasts was significantly inhibited in the experimental of group II(P<0.01). The gray value of the control group and the fluorine group were 85.89±7.90, 116.76±8.04, respectively.
Conclusions:
Fluoride might disturb the signal transduction between the epithelia and mesenchyma by inhibiting the expression of TGF-β3 in ameloblasts, which in turn may inhibit the differentiation and function of the tooth-forming cells.
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