Related Experiment Video
Updated: Feb 8, 2026

Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
Published on: July 3, 2025
First molecular epidemiological study of Mycobacterium avium subsp. paratuberculosis in cattle and buffalo from
Pedro Paulo Feitosa de Albuquerque1, Renata Pimentel Bandeira de Melo2, Marilene de Farias Brito3
1Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, PE, Brazil. ppfalbuquerque@gmail.com.
Abstract:
Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to carry out a study of the molecular epidemiology of the MAP using the restriction enzyme analysis (REA) technique of IS1311 MAP region in biological samples of feces, intestinal tissue, and mesenteric lymph nodes of cattle and buffaloes from six Brazilian states. In total, 109 samples of feces and tissues of cattle and buffaloes were collected from animal paratuberculosis suspected. Twenty-five samples were positive in the detection of the DNA of the IS900 region of MAP and it was possible to type 18 strains in the analysis of the region IS1311, being 100% of them identified as belonging to subtype Bison MAP strain. This is the first epidemiological molecular study of MAP in Brazil. The results indicate that paratuberculosis is widespread in cattle and in buffaloes in several regions of Brazil, and the subtype Bison MAP strain was the only one identified in the samples analyzed in this study, demonstrating the similarity between the strains from different states tested. These results provide the necessary support for the implementation of paratuberculosis control strategies in cattle and buffaloes in Brazil.
More Related Videos
06:57Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
Published on: May 12, 2023
06:27Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Related Concept Videos
Study Designs in Epidemiology
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
Confounding in Epidemiological Studies
Bias in Epidemiological Studies
Introduction to Epidemiology
Causality in Epidemiology
IR Frequency Region: Fingerprint Region