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Effect of trimetazidine on membrane damage induced by oxygen free radicals in human red cells
Abstract:
The effect of trimetazidine, 1-(2, 3, 4 trimethoxybenzyl)piperazine di-hydrochloride, on membrane damage induced by oxygen free radicals in red cells was studied in seven healthy volunteers after oral administration. Red cells collected prior to and after a 7 day treatment period with trimetazidine were incubated in the presence of phenazine methosulphate (an intracellular oxygen free radical generator) and diethyldithiocarbamate (a Cu-Zn superoxide dismutase inhibitor). The loss of intracellular K+ induced by oxygen free radicals and the membrane content of peroxidated lipids were significantly reduced in red cells collected after the period of treatment. These results indicate a potent antioxidant activity of trimetazidine which could explain its cardioprotective role during ischaemic and reperfusion phases in which oxygen free radicals are generated and probably implicated in the genesis of cardiac cell injury.
Insights
Trimetazidine demonstrated significant antioxidant activity by protecting red blood cells from oxygen free radical damage. This finding supports its potential cardioprotective effects in conditions involving oxidative stress.
Area of Science:
- Biochemistry
- Pharmacology
- Hematology
Background:
- Oxygen free radicals contribute to cellular damage.
- Red blood cell membranes are susceptible to oxidative injury.
- Trimetazidine is being investigated for its therapeutic properties.
Purpose of the Study:
- To evaluate the antioxidant effects of trimetazidine on red blood cells.
- To assess trimetazidine's impact on membrane damage induced by free radicals.
- To explore the mechanism behind trimetazidine's potential cardioprotective role.
Main Methods:
- Oral administration of trimetazidine to healthy volunteers for 7 days.
- Incubation of red blood cells with free radical generators and enzyme inhibitors.
- Measurement of intracellular potassium loss and peroxidated lipids in red cells.
Main Results:
- Trimetazidine significantly reduced intracellular potassium loss caused by oxygen free radicals.
- A significant decrease in membrane peroxidated lipids was observed after trimetazidine treatment.
- The drug exhibited potent antioxidant activity in red blood cells.
Conclusions:
- Trimetazidine possesses significant antioxidant properties.
- These antioxidant effects may contribute to trimetazidine's cardioprotective role during ischemia-reperfusion.
- The study provides evidence for trimetazidine's protective mechanism against oxidative stress-induced cell injury.