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Updated: Feb 8, 2026

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
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Development and validation of a functional cell-based neutralizing antibody assay for ipilimumab

Weifeng Xu1, Jennifer Cummings1, Michael Sank1

  • 1Analytical & Bioanalytical Operations, Bristol-Myers Squibb, Princeton, NJ 08543, USA.

Bioanalysis
|June 28, 2018
PubMed
Summary

Developing a cell-based assay for ipilimumab (a cancer immunotherapy) presented challenges in cell line construction and sample handling. Overcoming these issues enabled successful validation for clinical sample testing.

Keywords:
acid dissociationbioassaymonoclonal antibody therapeuticsneutralizing antibodysample pretreatment

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