Autophagy-Associated Proteins Control Ebola Virus Internalization Into Host Cells
Olena Shtanko1, Ann N Reyes1, William T Jackson2
1Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio.
The Journal of Infectious Diseases
|June 28, 2018
Summary
Autophagy proteins are crucial for Ebola virus (EBOV) entry into host cells. Microtubule-associated protein 1A/B light chain 3B (LC3B)-II specifically mediates EBOV-containing vesicle internalization.
Area of Science:
- Cell Biology
- Virology
- Molecular Biology
Background:
- Ebola virus (EBOV) entry into host cells occurs via macropinocytosis, a cellular process not fully understood.
- Autophagy, a cellular degradation pathway, shares components with macropinocytosis, suggesting a potential role in viral entry.
Purpose of the Study:
- To investigate the role of autophagy-associated proteins in EBOV macropinocytosis.
- To determine the specific contribution of microtubule-associated protein 1A/B light chain 3B (LC3B) in EBOV uptake.
Main Methods:
- Depletion of autophagy-associated proteins (beclin 1, ATG7, LC3B) in host cells.
- Assessment of EBOV uptake and macropinocytic vesicle formation.
- Analysis of LC3B-I and LC3B-II interaction with macropinocytic structures.
Main Results:
- Depletion of beclin 1, ATG7, or LC3B significantly inhibited EBOV uptake.
- Autophagy protein depletion blocked macropinocytic vesicle formation at the cell surface.
- LC3B-II was identified as critical for the internalization of macropinocytic vesicles containing EBOV.
Conclusions:
- Autophagy-associated proteins are essential for EBOV trafficking and cellular entry.
- LC3B-II plays a critical role in the internalization of macropinosomes, facilitating EBOV infection.
Related Concept Videos
00:50What are Viruses?
128.3K
Overview
128.3K
01:31Internal Receptors
74.7K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.7K
01:27Autophagy
5.9K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.9K
02:00Internal Energy
36.8K
The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
36.8K
01:29Internal Energy
7.1K
The internal energy of a thermodynamic system is the sum of the kinetic and potential energies of all the molecules or entities in the system. The kinetic energy of an individual molecule includes contributions due to its rotation and vibration, as well as its translational energy. The potential energy is associated only with the interactions between one molecule and the other molecules of the system. Neither the system's location nor its motion is of any consequence as far as the internal...
7.1K
01:28The Cell Cycle Control System
5.6K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.6K