Transcriptome analysis demonstrates that long noncoding RNA is involved in the hypoxic response in Larimichthys...

Area of Science:

• Aquatic biology
• Molecular biology
• Fish physiology

Background:

• Large yellow croaker (Larimichthys crocea) possess limited hypoxia tolerance.
• Hypoxia-inducible factor (HIF)-1α mRNA levels in the brain show minimal changes under hypoxic conditions.

Purpose of the Study:

• Investigate noncoding RNA's role in the hypoxic response of L. crocea.
• Identify long noncoding RNAs (lncRNAs) involved in hypoxia signaling.
• Elucidate the regulatory mechanisms of hypoxia stress in teleost fish.

Main Methods:

• Generated a catalog of brain lncRNAs from L. crocea under hypoxic stress.
• Analyzed lncRNA expression patterns and the HIF signaling pathway via RNA sequencing.
• Examined the relationship between lncRNAs and hypoxia-inducible genes.

Main Results:

• Identified a novel lncRNA, Linc_06633.1, upstream of Prolyl hydroxylase domain 2 (PHD2).
• Observed a significant increase in PHD2 expression after 6 and 12 hours of hypoxia.
• Linc_06633.1 is proposed to promote PHD2 expression under hypoxic conditions.

Conclusions:

• This is the first study to explore lncRNA regulation of the hypoxia signaling pathway in L. crocea.
• lncRNAs play a crucial role in the hypoxia stress response in teleost fish.
• A regulatory profile of L. crocea under hypoxia was constructed, highlighting lncRNA involvement.