Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

4.5K
A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.5K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

6.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

667
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
667

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Characterization of Hotspot Mutations in HER2, BRAF, KRAS, and PIK3CA in Canine Pulmonary Adenocarcinoma from Japan.

Veterinary sciences·2026
Same author

Case Report: Neurological and pulmonary angiostrongylosis in a Ryukyu long-furred rat (<i>Diplothrix legata</i>) on Amami-Oshima Island, Japan.

Frontiers in parasitology·2026
Same author

From pathology to phylogeny: Highly rearranged mitochondrial genome of the emerging testudine intranuclear coccidium.

International journal for parasitology·2026
Same author

Cutaneous T-Cell Lymphoma With Conjunctival T-Lymphocyte Infiltration in a Dog.

Veterinary ophthalmology·2026
Same author

Increased Infiltration of CD4<sup>+</sup>, CD8<sup>+</sup>, and CD68<sup>+</sup> Cells at the Invasive Front Is Associated With Favorable Prognosis in Obstructive Colorectal Cancer: A Retrospective Observational Study.

Cancer reports (Hoboken, N.J.)·2026
Same author

A first case of <i>Hepatozoon</i> infection in a wild Amami rabbit (<i>Pentalagus furnessi</i>) in Tokunoshima Island, Japan.

International journal for parasitology. Parasites and wildlife·2026

Related Experiment Video

Updated: Feb 8, 2026

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
07:29

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Published on: June 20, 2015

20.2K

Pancreatic cancer stem cells: features and detection methods.

Toshiyuki Ishiwata1, Yoko Matsuda2, Hisashi Yoshimura3

  • 1Division of Aging and Carcinogenesis, Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 173-0015, Japan. tishiwat@tmig.or.jp.

Pathology Oncology Research : POR
|June 28, 2018
PubMed
Summary

Pancreatic cancer stem cells (CSCs) drive tumor growth and treatment resistance. This review details methods for identifying these cells, crucial for developing targeted therapies and improving patient outcomes.

Keywords:
Cancer stem cell markerCancer stem cell/CSCPancreatic cancerSide populationSphere

More Related Videos

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
11:44

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

Published on: September 26, 2010

22.8K
In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K

Related Experiment Videos

Last Updated: Feb 8, 2026

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
07:29

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Published on: June 20, 2015

20.2K
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
11:44

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

Published on: September 26, 2010

22.8K
In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
07:32

In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

Published on: September 25, 2021

3.6K

Area of Science:

  • Oncology
  • Cancer Biology
  • Gastroenterology

Background:

  • Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer known for metastasis and recurrence.
  • Cancer stem cells (CSCs) are implicated in PDAC initiation, metastasis, and resistance to therapies.
  • Identifying and targeting PDAC CSCs is critical for improving patient prognosis.

Purpose of the Study:

  • To review and summarize common experimental methods for identifying cancer stem cells (CSCs) in pancreatic ductal adenocarcinoma (PDAC).
  • To discuss the role of CSCs in PDAC progression and therapeutic resistance.
  • To highlight the ongoing debate regarding specific CSC markers in PDAC.

Main Methods:

  • Review of literature on CSC detection methodologies in PDAC.
  • Discussion of CSC-specific marker detection (e.g., CD133, CD24, CD44, ALDH-1).
  • Explanation of sphere-formation assays and side-population (SP) cell detection.

Main Results:

  • Multiple methods exist for CSC identification in PDAC, including marker detection, sphere assays, and SP cell analysis.
  • Various markers are associated with PDAC CSCs, but their specificity and combinations require further clarification.
  • Understanding CSC characteristics is essential for developing effective PDAC treatments.

Conclusions:

  • Accurate identification of PDAC CSCs is vital for advancing treatment strategies.
  • Further research is needed to resolve controversies surrounding PDAC CSC markers.
  • Targeting CSCs holds promise for overcoming therapeutic resistance and improving PDAC patient survival.