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MiR-524 inhibits cell proliferation and induces cell apoptosis in thyroid cancer via targeting SPAG9

Z Zhen1, F Dong, H Shen

  • 1Department of Otolaryngology, Head and Neck Surgery, Peking University First Hospital, Beijing, China. bicuculline@gmail.com.

European Review for Medical and Pharmacological Sciences
|June 28, 2018
PubMed
Abstract

Insights

MicroRNA-524 (miR-524) is downregulated in thyroid cancer. Restoring miR-524 inhibits cancer cell proliferation and promotes apoptosis by targeting SPAG9.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Thyroid cancer is a prevalent endocrine malignancy.
  • Dysregulation of microRNAs (miRNAs) is implicated in thyroid cancer development.
  • The specific role of miR-524 in thyroid cancer remains to be fully elucidated.

Purpose of the Study:

  • To investigate the effect of miR-524 on thyroid cancer cell proliferation.
  • To elucidate the underlying molecular mechanism of miR-524 action in thyroid cancer.
  • To identify potential therapeutic targets related to miR-524.

Main Methods:

  • Quantitative Real-time polymerase chain reaction (qRT-PCR) to measure miR-524 expression.
  • Methyl thiazolyl tetrazolium (MTT) assays to assess cell proliferation.
  • Apoptosis assays and luciferase reporter assays to determine molecular mechanisms.

Main Results:

  • MiR-524 expression was significantly reduced in thyroid cancer tissues compared to adjacent normal tissues.
  • Overexpression of miR-524 suppressed thyroid cancer cell proliferation.
  • MiR-524 induced apoptosis in thyroid cancer cells.
  • SPAG9 was identified as a direct target gene of miR-524, with its expression negatively regulated by miR-524.

Conclusions:

  • MiR-524 acts as a tumor suppressor in thyroid cancer.
  • MiR-524 inhibits thyroid cancer cell proliferation and induces apoptosis.
  • The miR-524/SPAG9 axis represents a potential therapeutic strategy for thyroid cancer.

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