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MARSI: metabolite analogues for rational strain improvement
João G R Cardoso1, Ahmad A Zeidan2, Kristian Jensen1
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.
Bioinformatics (Oxford, England)
|June 29, 2018
Summary
Metabolite analogues (MAs) offer a novel approach for microbial strain improvement by targeting metabolic pathways. This method enables rewiring cellular metabolism without genetic modification, facilitating industrial applications.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Synthetic Biology
Background:
- Metabolite analogues (MAs) are crucial tools for isolating microbial mutants by inhibiting native metabolite utilization.
- Genome-scale metabolic models can predict the phenotypic effects of MAs on cellular metabolism.
Purpose of the Study:
- To introduce the Metabolite Analogues for Rational Strain Improvement (MARSI) framework.
- To provide a rational strategy for strain improvement by targeting metabolites instead of genes or reactions.
- To enable metabolic rewiring without recombinant DNA technology.
Main Methods:
- The MARSI framework identifies target metabolites for MA intervention.
- MARSI utilizes genome-scale metabolic models to predict MA effects.
- MARSI includes tools to identify candidate MAs from drug databases.
Main Results:
- MARSI offers a rational approach to strain improvement.
- MA implementation via culture media allows metabolic rewiring without genetic engineering.
- MARSI facilitates the identification of potential MAs for experimental use.
Conclusions:
- MARSI presents a DNA-free method for microbial strain improvement.
- The framework supports targeted metabolic engineering through metabolite analogue application.
- MARSI streamlines the discovery of MAs for industrial biotechnology.
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