Related Experiment Video

Updated: Feb 8, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.7K

iTRAQ-Based Proteomic Analysis reveals possible target-related proteins and signal networks in human osteoblasts

Tianyi Cai1, Baojin Wu1, Xinjie Tang1

  • 1Department of Plastic and Reconstructive Surgery, Huashan Hospital, Fudan University School of Medicine, No. 12, Wu Lu Mu Qi Road (M), Shanghai, 200040 China.

Proteome Science
|June 29, 2018
PubMed
Abstract

Insights

This study used proteomics to identify 149 differentially expressed proteins in FGFR2-overexpressing osteoblasts, revealing key pathways involved in bone formation and cell regulation.

Area of Science:

  • Molecular Biology
  • Proteomics
  • Bioinformatics

Background:

  • Fibroblast growth factor receptor 2 (FGFR2) is crucial for skeletogenesis.
  • The precise molecular mechanisms of FGFR2 in osteoblasts remain incompletely understood.

Purpose of the Study:

  • To investigate protein profile changes regulated by FGFR2 in human osteoblasts.
  • To elucidate the molecular mechanisms of FGFR2 function in osteogenesis.

Main Methods:

  • Established a human osteoblast cell line overexpressing FGFR2 using lentivirus vectors.
  • Employed isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS to analyze proteomic differences.
  • Utilized Gene Ontology (GO) and pathway analysis to identify key molecular pathways.

Main Results:

  • Identified 149 differentially expressed proteins (DEPs) between control and FGFR2-overexpressing cells.
  • Observed downregulation of Collagen I (ColI), Tenascin C (TNC), Fibronectin 1 (FN1), and CDKN1A; upregulation of UBE2E3, ADNP2, and HSP70.
  • KEGG pathway analysis highlighted significant alterations in cell death, PI3K-Akt signaling, focal adhesion, and cell cycle pathways.

Conclusions:

  • This is the first proteomic study to examine protein alterations in FGFR2-overexpressing osteoblasts.
  • Provides a comprehensive protein dataset regulated by FGFR2.
  • Offers insights into the potential molecular mechanisms of FGFR2 in human osteoblasts.
Keywords:
FGFR2 osteoblastiTRAQ

More Related Videos

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Protein Networks02:26

Protein Networks

2.9K
Small-Signal Analysis of MOSFET Amplifiers01:23

Small-Signal Analysis of MOSFET Amplifiers

In small-signal analysis, a MOSFET transistor amplifier acts as a linear amplifier when operating in its saturation region. The gate-to-source voltage (VGS) of the MOSFET is the sum of the DC biasing voltage and the small time-varying input signal. This combination sets up the operating point and modulates the drain current (ID) that flows from the drain to the source. When a small AC signal is superimposed on the DC bias voltage at the gate, the instantaneous drain current comprises three...
1.1K
Small-Signal Analysis of BJT Amplifiers01:21

Small-Signal Analysis of BJT Amplifiers

Small signal analysis is a fundamental approach used in electronics to understand how a Bipolar Junction Transistor (BJT) amplifier processes signals. In the active region, the BJT is designed for linear amplification. The transistor's behavior under these conditions is governed by its instantaneous base-emitter voltage VBE, a sum of the DC bias VBE, and a small AC signal VBE, resulting in the collector current iC. Here, the collector current has a DC component and an AC component.
1.8K
Endocrine Signaling01:45

Endocrine Signaling

Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
68.2K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K