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Updated: Aug 14, 2026

Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells
Published on: June 15, 2013
Effect of insulin on an insulin receptor of PLC/PRF/5 human hepatoma cell line
Abstract:
A human hepatoma cell line (PLC/PRF/5) contains several copies of hepatitis B virus (HBV) DNA in integrated form and releases hepatitis B surface antigen (HBsAg) in the form of 22 nm particles in culture medium; studies of the supernatant fluid failed to provide evidence of the morphologically intact virion (Dane particle) or hepatitis B infectivity. In this paper we describe the effect of insulin on cell growth and HBsAg production by these cells; moreover we describe the insulin binding to specific cell membrane receptors. Data in our hands point to a different insulin binding related to different incubation conditions.
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