Related Experiment Video
Updated: Feb 8, 2026

How to Build a Laser Speckle Contrast Imaging LSCI System to Monitor Blood Flow
Published on: November 11, 2010
Enhancing vascular visualization in laser speckle contrast imaging of blood flow using multi-focus image fusion
Wenzhi Lv1,2, Yang Wang1,2, Xiao Chen1,2
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Laser speckle contrast imaging (LSCI) is a full-field optical imaging method for monitoring blood flow and vascular morphology with high spatiotemporal resolution. However, due to the limited depth of field of optical system, it is difficult to capture a clear blood flow image with all blood vessels focused, especially for the non-planar biological tissues. In this study, a multi-focus image fusion method based on contourlet transform is introduced to reduce the misfocus effects in LSCI. The experimental results suggest that this method can provide an all-in-focus blood flow image, which is convenient to observe the blood vessels.
More Related Videos
06:24Paired Cisterna Magna Nanoinjection and Laser Speckle Contrast Imaging Assay to Study Cerebral Blood Flow Regulation In Vivo
Published on: July 8, 2025
07:20A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
Published on: August 30, 2017
Related Concept Videos
Imaging Studies VII: Vascular Imaging
Blood Flow
Nuclear Fusion
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
Autoregulation of Blood Flow
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
Applications of Integration to Find Blood Flow
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...