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RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells
Satoshi Arai1,2,3, Rókus Kriszt4, Kazuki Harada5
1Cell Signaling Group, Waseda Bioscience Research Institute in Singapore (WABIOS), Singapore, 138667, Republic of Singapore.
Angewandte Chemie (International Ed. in English)
|June 29, 2018
Summary
Scientists developed new multicolor fluorescent indicators to visualize cellular energy (ATP) dynamics within organelles. This breakthrough allows simultaneous tracking of ATP, cAMP, and Ca2+ in various cell types, advancing metabolism research.
Area of Science:
- Cellular Biology
- Biochemistry
- Metabolic Research
Background:
- Adenosine triphosphate (ATP) is crucial for cellular energy regulation.
- Visualizing ATP spatiotemporal dynamics in single cells and organelles is vital for understanding cellular metabolism.
- Current methods face challenges in visualizing ATP dynamics across distinct intracellular compartments and its interaction with other signaling molecules.
Purpose of the Study:
- To develop novel tools for visualizing subcellular ATP dynamics.
- To enable simultaneous monitoring of ATP in different cellular locations, such as the cytoplasm and mitochondria.
- To investigate the interplay between ATP, cAMP, and Ca2+ in cellular signaling.
Main Methods:
- Development of multicolor ATP indicators using single fluorescent proteins.
- Application of these indicators in mammalian, plant, and worm-derived cells.
- Integration with additional fluorescent indicators for simultaneous multi-analyte visualization.
Main Results:
- Successful simultaneous visualization of subcellular ATP dynamics in cytoplasm and mitochondria across diverse cell types.
- Demonstrated visualization of the dynamic interplay between ATP, cAMP, and Ca2+ in activated brown adipocytes.
- Established a new set of versatile indicator tools for studying cellular energy metabolism.
Conclusions:
- The developed multicolor ATP indicators offer unprecedented capabilities for spatiotemporal analysis of cellular energy.
- These tools facilitate the study of energy metabolism and signaling crosstalk in various biological contexts.
- This research provides a foundation for future investigations into cellular energy dynamics and regulation.
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