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Updated: Feb 8, 2026

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
Published on: June 15, 2018
Improving selectivity and sensitivity of protein quantitation by LC-HR-MS/MS: determination of somatropin in rat
Peter Bults1,2, Marcel Meints1, Anders Sonesson3
1Bioanalytical Laboratory, PRA Health Sciences, Amerikaweg 18, 9407 TK, Assen, The Netherlands.
Aim:
Protein quantitation by digestion of a biological sample followed by LC-MS analysis of a signature peptide can be a challenge because of the high complexity of the digested matrix. Results/methodology: The use of LC with high-resolution (quadrupole-TOF) MS detection allowed quantitation of the 22-kDa biopharmaceutical somatropin in 60 μl of rat plasma down to 25 ng/ml with minimal further sample treatment. Reducing the mass extraction window to 0.01 Da considerably decreased the interference of tryptic peptides, enhanced sensitivity and improved accuracy and precision. Analysis with LC-MS/MS resulted in a less favorable limit of quantitation of 100 ng/ml.
Conclusion:
HRMS is an interesting option for the quantitation of proteins after digestion and has the potential to improve sensitivity with minimal method development.
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