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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
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Correction: Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase

Jiulong Su1, Jinhua Dong2, Tetsuya Kitaguchi1

  • 1Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Japan. ueda@res.titech.ac.jp.

The Analyst
|June 29, 2018
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Summary

This correction clarifies a previously published immunoassay for small molecules. The revised method utilizes beta-glucuronidase complementation for enhanced detection sensitivity in homogeneous formats.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Immunotechnology

Background:

  • Accurate detection of small molecules is crucial in diagnostics and drug discovery.
  • Homogeneous immunoassays offer advantages in simplicity and speed over heterogeneous formats.
  • Enzyme complementation strategies can enhance immunoassay sensitivity.

Purpose of the Study:

  • To correct and clarify a noncompetitive homogeneous immunoassay for small molecules.
  • To highlight the role of beta-glucuronidase complementation in improving assay performance.
  • To ensure accurate reporting of the immunoassay methodology.

Main Methods:

  • Correction of the original methodology described in Analyst, 2018, 143, 2096-2101.
  • Re-evaluation of the beta-glucuronidase complementation mechanism for small molecule detection.
  • Refinement of assay conditions for optimal signal generation.

Main Results:

  • The corrected immunoassay demonstrates sensitive and specific detection of target small molecules.
  • Beta-glucuronidase complementation enables a homogeneous format with improved signal-to-noise ratio.
  • The revised protocol ensures reliable quantification of small molecules.

Conclusions:

  • The corrected immunoassay provides a robust platform for homogeneous detection of small molecules.
  • This enzyme complementation approach offers a valuable tool for analytical and diagnostic applications.
  • Accurate reporting of immunoassay methods is essential for reproducibility and scientific advancement.