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Related Experiment Video

Updated: Feb 8, 2026

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

Intracellular Delivery via Noncharged Sequence-Defined Cell-Penetrating Oligomers.

Ngoc N Phan¹, Connie Li¹, Christopher A Alabi¹

¹Robert F. Smith School of Chemical and Biomolecular Engineering , Cornell University , 120 Olin Hall , Ithaca , New York 14853 , United States.

Bioconjugate Chemistry

|June 29, 2018

Summary

Researchers discovered new noncharged cell-penetrating oligoTEAs (CPOTs) for efficient intracellular drug delivery. These CPOTs show improved serum stability and lower cytotoxicity compared to traditional cell-penetrating peptides (CPPs).

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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

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Published on: November 7, 2013

Area of Science:

Biochemistry
Nanotechnology
Drug Delivery

Background:

Intracellular drug delivery systems face challenges with serum stability and efficiency.
Traditional cell-penetrating peptides (CPPs) offer cargo delivery but suffer from degradation and unwanted biological interactions.
Cationic CPPs have limitations hindering their therapeutic use.

Purpose of the Study:

To discover a new class of noncharged cell-penetrating oligoTEAs (CPOTs).
To evaluate the efficiency and cytotoxicity of CPOTs for intracellular delivery.
To compare CPOT performance against established CPPs like R9 peptide.

Main Methods:

Discovery and synthesis of novel noncharged oligoTEAs (CPOTs).
In vitro assessment of cellular entry efficiency across various cell lines.
Evaluation of cytotoxicity and serum stability.
Comparative analysis with a benchmark CPP (R9 peptide).

Main Results:

CPOTs demonstrated extensive and rapid cellular entry across different cell lines.
CPOTs exhibited significantly lower cytotoxicity compared to traditional CPPs.
The new noncharged CPOTs outperformed the R9 peptide in delivery efficiency.
CPOTs showed improved stability in biological environments.

Conclusions:

A novel class of noncharged cell-penetrating oligoTEAs (CPOTs) has been successfully developed.
CPOTs represent a promising advancement for intracellular drug delivery, overcoming limitations of cationic CPPs.
These noncharged macromolecular transporters offer a safer and more effective alternative for delivering hydrophilic small-molecule therapeutics intracellularly.