Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A beginner's guide to rigor and reproducibility in fluorescence imaging experiments.

Jen-Yi Lee1, Maiko Kitaoka2

  • 1Molecular Imaging Center, Cancer Research Laboratory, University of California, Berkeley, Berkeley, CA 94720.

Molecular Biology of the Cell
|June 29, 2018
PubMed
Summary

This perspective offers a guide to best practices in fluorescence light microscopy for reliable cell biology research. It emphasizes critical assessment of experimental design, execution, and data management for rigor and reproducibility.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fixation and Expansion Microscopy of <i>Xenopus</i> Egg Extract Spindles.

Bio-protocol·2025
Same author

Optimized expansion microscopy reveals species-specific spindle microtubule organization in <i>Xenopus</i> egg extracts.

Molecular biology of the cell·2025
Same author

Optimized expansion microscopy reveals species-specific spindle microtubule organization in <i>Xenopus</i> egg extracts.

bioRxiv : the preprint server for biology·2024
Same author

Running the gauntlet: challenges to genome integrity in spermiogenesis.

Nucleus (Austin, Tex.)·2024
Same author

Preparation of Xenopus borealis and Xenopus tropicalis Egg Extracts for Comparative Cell Biology and Evolutionary Studies.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Molecular conflicts disrupting centromere maintenance contribute to Xenopus hybrid inviability.

Current biology : CB·2022

Area of Science:

  • Cell Biology
  • Microscopy
  • Biotechnology

Background:

  • Fluorescence light microscopy is crucial for studying cellular mechanisms.
  • Advanced tools like fluorescent proteins and superresolution enhance microscopy's power.
  • Ensuring reliability and reproducibility in imaging experiments is essential.

Purpose of the Study:

  • To provide a guide on best practices for designing and executing fluorescence imaging experiments.
  • To introduce researchers to concepts for acquiring rigorous imaging data.
  • To promote critical assessment of bias, rigor, and reproducibility in all experimental steps.

Main Methods:

  • Review of experimental design considerations in fluorescence microscopy.
  • Guidelines for execution of imaging experiments.

Related Experiment Videos

  • Recommendations for data analysis, reporting, and management.

    • Main Results:

    • A framework for best practices in fluorescence light microscopy is presented.
    • Emphasis on critical evaluation of each experimental step for rigor.
    • Empowering researchers to achieve reproducible imaging data.

    Conclusions:

    • Adherence to best practices is vital for reliable fluorescence microscopy.
    • Critical assessment across all stages ensures data integrity.
    • This guide aims to enhance the rigor of cell biology imaging studies.