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Updated: Feb 8, 2026

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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Rapid and Versatile Cell Aggregate Formation Using Lipid-Conjugated Heparin.

Eunsol Kim1, Jong Chul Kim1, Kiyoon Min1

  • 1School of Materials Science and Engineering , Gwangju Institute of Science and Technology , 123 Cheomdan-gwagiro , Buk-gu, Gwangju 61005 , Republic of Korea.

ACS Applied Materials & Interfaces
|June 29, 2018
PubMed
Summary

Researchers developed a novel method for creating diverse cell aggregates using lipid-conjugated heparin. This rapid technique produces viable cell aggregates of various shapes and sizes for therapeutic applications.

Keywords:
cell surface engineeringcell threadinstant cell aggregationlipid-conjugated heparinmulticompartment

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Tissue Engineering

Background:

  • Cell aggregates are crucial for cell analysis, tissue models, and regenerative medicine.
  • Conventional methods for aggregate formation are time-consuming and yield limited shapes.

Purpose of the Study:

  • To develop a rapid and versatile method for generating diverse cell aggregates.
  • To explore the use of lipid-conjugated heparin in cell aggregate formation.

Main Methods:

  • Utilized lipid-conjugated heparin in cell suspensions.
  • Employed a brief shaking incubation (approx. 30 min).
  • Investigated template-guided and template-free aggregate formation.

Main Results:

  • Successfully formed viable cell aggregates of various sizes and shapes.
  • Demonstrated applicability across multiple cell types (stem cells, fibroblasts, T lymphocytes).
  • Achieved template-modulated and template-free aggregate structures (e.g., tube-shaped).

Conclusions:

  • Lipid-conjugated heparin offers a rapid, adaptable approach to cell aggregate production.
  • This method enhances possibilities for cell therapy and tissue engineering applications.
  • The formed cell aggregates retain viability and biological function.