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Related Experiment Videos

Circulating activated B cells in primary biliary cirrhosis.

S P James, E A Jones, J H Hoofnagle

    Journal of Clinical Immunology
    |July 1, 1985
    PubMed
    Summary

    Patients with primary biliary cirrhosis (PBC) show increased immunoglobulin-secreting cells, but their B cells are hyporesponsive to polyclonal activators. This suggests specific B cell subpopulations, not generalized hyperactivity, cause humoral immune abnormalities in PBC.

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    Area of Science:

    • Immunology
    • Hepatology
    • Autoimmune Diseases

    Background:

    • Primary biliary cirrhosis (PBC) is associated with humoral immune system dysfunction.
    • Understanding B cell behavior in PBC is crucial for explaining immune abnormalities.

    Purpose of the Study:

    • To investigate B cell activation and responsiveness in patients with primary biliary cirrhosis (PBC).
    • To determine if B cells from PBC patients show evidence of activation and can be stimulated by polyclonal activators.

    Main Methods:

    • Utilized a reverse hemolytic plaque assay to quantify immunoglobulin-secreting cells.
    • Assessed B cell activation by measuring transferrin receptor expression.
    • Stimulated lymphocytes with pokeweed mitogen and Epstein-Barr virus (EBV) to evaluate immunoglobulin production.

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  • Determined B cell numbers using anti-Leu 12 antibody and assessed T-cell suppressor function.

    • Main Results:

    • PBC patients exhibited a significant increase in circulating immunoglobulin-secreting cells compared to controls and hepatitis B virus (HBV) infected patients.
    • Less than 1% of circulating B cells in PBC patients showed signs of activation, with no increased transferrin receptor expression in most.
    • Immunoglobulin production upon stimulation with pokeweed mitogen and EBV was reduced in PBC patients.
    • This hyporesponsiveness was not linked to reduced B cell numbers or increased suppressor T-cell activity.

    Conclusions:

    • Humoral abnormalities in PBC are likely caused by the activation of a small B cell subpopulation.
    • PBC does not involve generalized B-cell hyperactivity.
    • Findings suggest a targeted immune response rather than a widespread B cell issue in PBC.