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Updated: Feb 8, 2026

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
Published on: July 6, 2010
Toll-like receptors 4, 5, 6 and 7 are constitutively expressed in non-human primate retinal neurons
Monica M Sauter1, Aaron W Kolb1, Curtis R Brandt2
1Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53706, United States.
Abstract:
The purpose of this study was to characterize cell-specific expression patterns of Toll-like receptors (TLR) in non-human primate (NHP) neural retina tissue. TLR 4, 5, 6, and 7 proteins were detected by immunblotting of macaque retina tissue lysates and quantitative PCR (qPCR) demonstrated TLRs 4-7 mRNA expression. Immunofluorescence (IF) microscopy detected TLRs 4-7 in multiple cell types in macaque neural retina including Muller, retinal ganglion cells (RGC), amacrine, and bipolar cells. These results demonstrate that TLRs 4-7 are constitutively expressed by neurons in the NHP retina raising the possibility that these cells could be involved in retinal innate inflammatory responses.
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