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Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice
Published on: July 5, 2013
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A method for single-neuron chronic recording from the retina in awake mice
Guosong Hong1, Tian-Ming Fu1, Mu Qiao2
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Science (New York, N.Y.)
|June 30, 2018
Summary
Researchers developed a nonsurgical method for stable, long-term in vivo recordings of retinal ganglion cells (RGCs) in mice. This breakthrough enables detailed study of neural circuitry and visual processing in awake animals.
Area of Science:
- Neuroscience
- Ophthalmology
- Biomedical Engineering
Background:
- The retina is a key model for neural circuitry research.
- Chronic in vivo electrophysiology of the retina has been challenging.
- Previous methods were limited in stability and invasiveness.
Purpose of the Study:
- To develop a nonsurgical method for stable chronic in vivo electrophysiology of retinal ganglion cells (RGCs).
- To enable long-term monitoring of RGC activity in awake, behaving animals.
- To investigate neural circuit function and circadian rhythms in the retina.
Main Methods:
- A novel noncoaxial intravitreal injection technique was developed.
- Mesh electronics were designed to unroll and conformally coat the retina.
- 16-channel electrophysiological recordings were performed in awake mice.
Main Results:
- The method achieved chronically stable in vivo recordings from single RGCs for over 2 weeks.
- Recordings captured stable responses to visual stimuli.
- Circadian rhythms in RGC responses were observed across multiple day/night cycles.
Conclusions:
- The nonsurgical intravitreal injection of mesh electronics provides a stable platform for chronic in vivo retinal recordings.
- This technique overcomes previous limitations in studying retinal neural circuitry.
- It opens new avenues for understanding visual processing and circadian regulation in the retina.
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