Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Instability of digoxin in acid medium using a nonisotopic method.

S A Khalil, S El-Masry

    Journal of Pharmaceutical Sciences
    |October 1, 1978
    PubMed
    Summary

    Digoxin hydrolysis rates were studied using a selective xanthydrol assay. Acidic conditions significantly impact digoxin stability, with faster dissolving tablets showing increased hydrolysis in USP medium.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preparation of new modified CuFe₂O₄ nanoparticles by benzalkonium chloride as enhancer of natural gas hydrate formation.

    Scientific reports·2026
    Same author

    Innovative role of Benzalkonium chloride as a quaternary ammonium salt for natural gas hydrate formation and storage.

    Scientific reports·2025
    Same author

    Laparoscopic intersphincteric resection for low rectal cancer - a video vignette.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2018
    Same author

    Laparoscopic total mesorectal excision for rectal cancer - a video vignette.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2016
    Same author

    Liberal perioperative fluid administration is an independent risk factor for morbidity and is associated with longer hospital stay after rectal cancer surgery.

    Annals of the Royal College of Surgeons of England·2016
    Same author

    Potential effect of Olea europea leaves, Sonchus oleraceus leaves and Mangifera indica peel extracts on aromatase activity in human placental microsomes and CYP19A1 expression in MCF-7 cell line: Comparative study.

    Cellular and molecular biology (Noisy-le-Grand, France)·2016

    Area of Science:

    • Pharmaceutical Chemistry
    • Analytical Chemistry
    • Drug Stability Studies

    Background:

    • Digoxin is a cardiac glycoside used to treat heart failure and atrial fibrillation.
    • Accurate quantification of digoxin is crucial for therapeutic efficacy and safety.
    • Existing analytical methods may be susceptible to interference, impacting stability assessments.

    Purpose of the Study:

    • To investigate digoxin hydrolysis rates under varying acidic conditions.
    • To compare the specificity of spectrofluorometric and xanthydrol methods for digoxin analysis.
    • To evaluate the impact of dissolution media on digoxin stability in tablet formulations.

    Main Methods:

    • A selective nonisotopic assay using a xanthydrol reagent was employed.
    • Digoxin hydrolysis rates were measured at 37°C across a pH range of 1.1-2.2.
    • Comparison of digoxin stability in British Pharmacopoeia (BP) water and United States Pharmacopeia (USP) 0.6% HCl dissolution media.

    Main Results:

    • Digoxin hydrolysis followed specific acid hydrolysis kinetics, with rate constants (K) ranging from 0.0357 to 0.0027 min⁻¹.
    • The spectrofluorometric method showed digoxigenin interference, unlike the specific xanthydrol method.
    • In USP medium (pH 1.3), the xanthydrol method indicated ~74% hydrolysis, while the fluorometric method showed none.
    • Fast-dissolving digoxin tablets exhibited greater hydrolysis than slow-dissolving ones in USP medium.

    Conclusions:

    • The xanthydrol assay provides a specific and reliable method for assessing digoxin hydrolysis.
    • Digoxin stability is significantly affected by acidic dissolution media, particularly in the USP formulation.
    • Dissolution characteristics of digoxin tablets influence their susceptibility to hydrolysis.

    Related Experiment Videos