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Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy.

Takayuki Uchihashi1, Hiroki Watanabe2, Noriyuki Kodera3

  • 1Nagoya, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Aichi, Japan. uchihast@d.phys.nagoya-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|June 30, 2018
PubMed
Summary

High-speed atomic force microscopy (HS-AFM) allows direct visualization of protein dynamics in liquid. Proper substrate preparation is crucial for successful HS-AFM imaging of biological molecules.

Keywords:
Biological moleculesHigh-speed AFMImagingSubstrate

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Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Biology

Background:

  • Recent advancements in high-speed atomic force microscopy (HS-AFM) facilitate direct observation of protein dynamics.
  • Successful HS-AFM imaging in liquid conditions depends critically on substrate selection.
  • Molecule adsorption onto substrates must preserve orientation, dynamics, and physiological function.

Purpose of the Study:

  • To describe protocols for preparing substrates used in HS-AFM.
  • To provide examples of HS-AFM observations of biological molecule dynamics.

Main Methods:

  • Detailed protocols for substrate preparation tailored for HS-AFM.
  • Application of prepared substrates for HS-AFM imaging of biological molecules.

Main Results:

  • Demonstration of effective substrate preparation techniques for HS-AFM.
  • Successful visualization of dynamic processes in biological molecules using HS-AFM.

Conclusions:

  • Appropriate substrate preparation is essential for high-resolution HS-AFM studies of molecular dynamics.
  • HS-AFM is a powerful tool for investigating the functional dynamics of proteins in their native-like liquid environment.