Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lattice Centering and Coordination Number02:33

Lattice Centering and Coordination Number

11.7K
The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1.
Types of Unit Cells
Imagine taking a large number of identical...
11.7K
Trends in Lattice Energy: Ion Size and Charge02:54

Trends in Lattice Energy: Ion Size and Charge

26.8K
An ionic compound is stable because of the electrostatic attraction between its positive and negative ions. The lattice energy of a compound is a measure of the strength of this attraction. The lattice energy (ΔHlattice) of an ionic compound is defined as the energy required to separate one mole of the solid into its component gaseous ions. For the ionic solid sodium chloride, the lattice energy is the enthalpy change of the process:
26.8K
Bewley Lattice Diagram01:12

Bewley Lattice Diagram

1.5K
The Bewley lattice diagram, developed by L. V. Bewley, effectively organizes the reflections occurring during transmission-line transients. It visually represents how voltage waves propagate and reflect within a transmission line, making it easier to understand the complex interactions that occur.
1.5K
Light as Energy01:35

Light as Energy

96.1K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
96.1K
Flow Sheet01:17

Flow Sheet

2.9K
Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:
2.9K
Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide absolute quantification of chromatin looping.

Nature structural & molecular biology·2026
Same author

Live-cell imaging of enhancer-promoter dynamics reveals transient contact-driven gene activation.

bioRxiv : the preprint server for biology·2026
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's disease.

Nature communications·2026
Same author

Decoding the <i>BRCA2</i> reversion principles underlying PARP inhibitor resistance.

bioRxiv : the preprint server for biology·2026
Same author

Integrated MINFLUX tracking reveals two distinct chromatin dynamics classes across cell types.

Nature structural & molecular biology·2026
Same author

World Ocean Database 2023: A Foundational Data Resource for and by the Global Ocean and Coastal Communities.

Scientific data·2026

Related Experiment Video

Updated: Feb 8, 2026

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.6K

Single Molecule Imaging in Live Embryos Using Lattice Light-Sheet Microscopy.

Mustafa Mir1, Armando Reimer2, Michael Stadler1

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 30, 2018
PubMed
Summary
This summary is machine-generated.

Live-cell single molecule imaging now allows observing transcription factor dynamics in thick specimens like embryos. This new lattice light-sheet microscopy protocol enables studying DNA-binding molecule behavior in vivo.

Keywords:
Drosophila melanogasterLattice light-sheet microscopyLive embryo imagingSelective plane illumination microscopySingle molecule fluorescenceSingle molecule imagingSingle molecule kineticsSingle particle trackingTranscription factor dynamics

More Related Videos

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy &#8212; Techniques for Imaging and Image Reconstruction
08:33

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction

Published on: July 19, 2024

1.4K
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

11.1K

Related Experiment Videos

Last Updated: Feb 8, 2026

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.6K
Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy &#8212; Techniques for Imaging and Image Reconstruction
08:33

Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction

Published on: July 19, 2024

1.4K
Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos
10:15

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

11.1K

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Live-cell single molecule imaging has revealed insights into DNA-binding molecule dynamics.
  • Previous studies were limited to thin cell cultures due to technological constraints.
  • Observing molecular behavior in thicker biological specimens like embryos remained challenging.

Purpose of the Study:

  • To establish a general procedure for single molecule imaging in living Drosophila melanogaster embryos.
  • To enable direct observation of transcription factor diffusion and binding dynamics in vivo.
  • To demonstrate the adaptability of the protocol for other thick samples, such as mouse embryos.

Main Methods:

  • Utilized lattice light-sheet microscopy for high-resolution imaging in living specimens.
  • Developed and applied a protocol for single molecule imaging in Drosophila melanogaster embryos.
  • Extended the methodology to live mouse embryos to showcase broader applicability.

Main Results:

  • Successfully implemented single molecule imaging in living Drosophila melanogaster embryos.
  • Enabled direct observation and analysis of transcription factor diffusion and binding dynamics.
  • Demonstrated the protocol's versatility by applying it to live mouse embryos.

Conclusions:

  • Lattice light-sheet microscopy overcomes previous limitations, enabling single molecule imaging in thick embryos.
  • The developed protocol facilitates the study of DNA-binding molecule dynamics in vivo.
  • This technique has broad implications for understanding gene regulation in complex biological systems.