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Related Concept Videos

What is the Cell Cycle?00:56

What is the Cell Cycle?

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The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the...
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What is the Cell Cycle?01:04

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The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
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The Cell Cycle Control System01:28

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The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
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The Cell Cycle Control System02:11

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The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
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Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
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Neural Regulation01:37

Neural Regulation

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Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
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Related Experiment Video

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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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Tracking neural crest cell cycle progression in vivo.

Sriivatsan G Rajan1, Kristin L Gallik1, James R Monaghan2

  • 1Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, 60607.

Genesis (New York, N.Y. : 2000)
|June 30, 2018
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Summary

Researchers developed a new transgenic zebrafish tool to track neural crest stem cell behavior. This method allows live, high-resolution monitoring of cell cycle progression during migration and differentiation.

Keywords:
FucciSox10zebrafish

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Genetics

Background:

  • Analyzing cell cycle dynamics is crucial for understanding stem cell behavior, including propagation, maintenance, and differentiation.
  • The neural crest, a transient stem cell population in vertebrate embryos, exhibits complex collective migration and differentiation patterns.
  • Traditional methods like immunohistochemistry offer limited spatiotemporal resolution for tracking dynamic cell populations like the neural crest.

Purpose of the Study:

  • To develop a novel tool for live, high-resolution tracking of cell cycle progression in specific embryonic stem cell populations.
  • To overcome the limitations of ubiquitous cell cycle reporters by creating a targeted system for neural crest cells.
  • To validate a new transgenic zebrafish line for studying neural crest development.

Main Methods:

  • Generation of a transgenic zebrafish line, Tg(-4.9sox10:mAG-gmnn(1/100)-2A-mCherry-cdt1(1/190)), enabling specific expression of the Fucci system in neural crest cells.
  • Utilizing the fluorescent, ubiquitination-based cell cycle indicator (Fucci) system for in vivo cell cycle readout.
  • Live, high-resolution imaging techniques to observe cell cycle progression in neural crest and derivative populations.

Main Results:

  • Successful generation and validation of a novel transgenic zebrafish line for targeted Fucci expression in neural crest cells.
  • Demonstration of the tool's capability for live, high-resolution tracking of cell cycle dynamics in migrating and differentiating neural crest cells.
  • The new system provides improved spatiotemporal insights into neural crest cell behavior compared to traditional methods.

Conclusions:

  • The developed transgenic zebrafish line is an effective tool for studying neural crest cell cycle dynamics.
  • This innovation enables detailed live imaging of cell cycle progression in specific stem cell populations during development.
  • The tool facilitates fundamental insights into stem cell biology, particularly concerning migration and differentiation processes.