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Related Experiment Video

Updated: Feb 8, 2026

Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline

Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline

Published on: January 30, 2019

Piscinibacter caeni sp. nov., isolated from activated sludge.

Dong-Zhi Chen¹, Ning-Ning Yu¹, Qi-Ying Chu¹

¹1College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.

International Journal of Systematic and Evolutionary Microbiology

|June 30, 2018

Summary

A novel bacterial strain, Piscinibacter caeni MQ-18^T, was identified from pharmaceutical factory activated sludge. This yellowish-pigmented bacterium represents a new species within the Piscinibacter genus, expanding our understanding of microbial diversity in industrial wastewater.

Keywords:

Piscinibacter caeni activated sludge new species

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Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

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Published on: October 15, 2015

Area of Science:

Microbiology
Bacteriology
Environmental Science

Background:

Activated sludge from pharmaceutical factories harbors diverse microbial communities.
Identification and characterization of novel bacterial species are crucial for understanding microbial ecosystems and potential biotechnological applications.

Purpose of the Study:

To isolate and characterize a novel bacterial strain from pharmaceutical factory activated sludge.
To determine the phylogenetic and taxonomic position of the novel strain within the genus Piscinibacter.

Main Methods:

Polyphasic taxonomy approach, including 16S rRNA gene sequence analysis and DNA-DNA hybridization.
Phenotypic characterization: Gram staining, motility, growth conditions (temperature, salinity, pH), and cellular fatty acid composition.
Chemotaxonomic analysis: quinone type, polyamines, polar lipids, and DNA G+C content determination.

Main Results:

A yellowish-pigmented, Gram-negative, aerobic, motile rod strain, designated MQ-18^T, was isolated.
16S rRNA gene sequence analysis showed high similarity to Piscinibacter species, but DNA-DNA relatedness values were low (<35.4%).
Physiological and chemotaxonomic analyses supported the distinctiveness of strain MQ-18^T, with optimal growth at 30°C, 0% NaCl, and pH 7.0. Predominant fatty acids included C16:0 and summed feature 3. DNA G+C content was 70.1%.

Conclusions:

Strain MQ-18^T represents a novel species of the genus Piscinibacter.
The proposed name for this new species is Piscinibacter caeni sp. nov.
The type strain is MQ-18^T (CCTCC AB 2017223^T=JCM 32138^T).