Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Mitotic Spindle02:27

The Mitotic Spindle

8.1K
The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
8.1K
Spindle Assembly02:50

Spindle Assembly

4.3K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.3K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
Polymer Classification: Architecture01:14

Polymer Classification: Architecture

3.9K
Polymers are classified as linear or branched on the basis of their chain architecture. The polymer chains in linear polymers have a long chain-like structure with minimal to no branching at all. Even if a polymer features large substituent groups on the monomer, which appear as branches to the skeleton, it is not considered a branched polymer. A branched polymer contains secondary polymer chains that arise from the main polymer chain. The branching occurs when the polymer growth shifts from...
3.9K
Dimensional Analysis03:40

Dimensional Analysis

65.0K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
65.0K
Dimensional Analysis01:27

Dimensional Analysis

682
Dimensional analysis is a valuable technique in fluid mechanics for simplifying complex problems by reducing them into dimensionless groups. These groups capture the essential relationships between the variables involved, allowing researchers and engineers to analyze fluid flow without dealing with each variable individually. This approach reduces the number of independent variables, allowing for easier analysis and better understanding of physical phenomena.
In fluid mechanics, dimensional...
682

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Visualizing suborganellar lipid distribution using correlative light and electron microscopy.

Nature cell biology·2026
Same author

Semi-automatic geometrical reconstruction and analysis of filopodia dynamics in 4D two-photon microscopy images.

BMC bioinformatics·2026
Same author

Human mitotic spindles as active liquid crystals: From collective behaviors to discrete filaments.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity.

bioRxiv : the preprint server for biology·2026
Same author

Mitochondrial Targeting by Elamipretide Improves Myocardial Bioenergetics Without Translating into Functional Benefits in HFpEF.

International journal of molecular sciences·2026
Same author

Compartment-specific transcriptome of motor neurons reveals impaired extracellular matrix signaling and activated cell cycle kinases in FUS-ALS.

Neurobiology of disease·2026
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: Feb 8, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

411.8K

Quantification of three-dimensional spindle architecture.

Norbert Lindow1, Stefanie Redemann2, Florian Brünig1

  • 1Zuse Institute Berlin, Berlin, Germany.

Methods in Cell Biology
|June 30, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed new techniques to analyze the 3D structure of cell division spindles. This enables quantitative measurement of microtubule properties for better understanding chromosome segregation.

Keywords:
3D reconstructionElectron microscopyElectron tomographyMeiosisMicrotubule tracingMicrotubulesMitosisSpindle structure

More Related Videos

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
16:59

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Published on: March 4, 2011

12.7K
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
12:38

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Published on: December 10, 2017

9.1K

Related Experiment Videos

Last Updated: Feb 8, 2026

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

411.8K
Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture
16:59

Computer-assisted Large-scale Visualization and Quantification of Pancreatic Islet Mass, Size Distribution and Architecture

Published on: March 4, 2011

12.7K
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
12:38

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Published on: December 10, 2017

9.1K

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biophysics

Background:

  • Mitotic and meiotic spindles are crucial microtubule-based structures for accurate chromosome segregation during cell division.
  • Electron tomography is the primary method for analyzing the 3D architecture of these spindles.
  • Previous work established methods for automatic segmentation and stitching of microtubules for large-scale reconstructions.

Purpose of the Study:

  • To present a comprehensive set of techniques for quantifying spindle parameters from 3D tomographic reconstructions.
  • To enable detailed analysis of specific microtubule classes within spindle structures.
  • To provide tools applicable to diverse spindle reconstructions across different organisms.

Main Methods:

  • Development of novel software and techniques for quantitative analysis of microtubule structures.
  • Application of established electron tomography methods for 3D spindle reconstruction.
  • Segmentation and stitching of microtubules from serial sections for large-scale data.

Main Results:

  • A comprehensive suite of techniques for quantifying spindle parameters has been established.
  • The methods allow for quantitative analysis of specific microtubule populations within spindles.
  • The techniques are validated for applicability to various spindle reconstructions.

Conclusions:

  • The developed techniques represent a significant advancement in analyzing spindle architecture.
  • Quantitative analysis of spindle parameters provides deeper biological insights into chromosome segregation.
  • These methods offer a versatile platform for structural studies of spindles in different biological systems.