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Updated: Feb 8, 2026

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Published on: October 28, 2022
Wide-field Choroidal Vascularity in Healthy Eyes
Sumit Randhir Singh1, Alessandro Invernizzi2, Mohammed Abdul Rasheed1
1Smt. Kanuri Santhamma Centre for Vitreo-Retinal Diseases, L V Prasad Eye Institute, Hyderabad, India.
Purpose:
To report variation of choroidal vascularity index (CVI) in macular and mid-equator areas in healthy subjects using wide-field optical coherence tomography (WF-OCT).
Design:
Prospective, cross-sectional study.
Methods:
Setting: Two-center study.
Study Population:
Twenty eyes of 20 healthy subjects.
Observation Procedure:
Single high-definition scans passing through the fovea in both vertical and horizontal meridians were studied. Images were taken in primary gaze and extremes of gaze and a manual montage was created. A previously reported semi-automated algorithm was used to calculate the CVI in macular, superior, inferior, temporal, and nasal quadrants.
Main Outcome Measures:
Difference in CVI in macular area and 4 quadrants.
Results:
Twenty eyes from 20 subjects were enrolled in the study. The mean age was 28.85 ± 6.29 years with men comprising 9 of the 20 subjects (45.0%). The refractive error (spherical equivalent) ranged from -3.00 to +0.75 diopters. The average CVI in the macular area (40.01 ± 7.67) was significantly smaller than in any of the other fundus areas (all P < .01). The maximum CVI was seen in the nasal quadrant (50.84 ± 5.64), followed by inferior (47.93 ± 9.31), temporal (46.14 ± 7.06), and superior (45.72 ± 7.69). The nasal quadrant was found to have the least coefficient of variation (CV) of CVI (0.11) while the inferior and macular area had the highest CV (0.19).
Conclusions:
We report CVI in WF-OCT in healthy young individuals. CVI seems to have a wide topographic variation. The macular area had the least CVI. CVI values are more consistent with lesser CV in the nasal quadrant compared to the other quadrants and macular area.
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